1. Important things to consider when purchasing a multimode microplate reader. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. Microseal 384-Well PCR Plates. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. When the user applies a set temperature of 37°C, the top is heated to 37. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. A = 2 - log 10 (%T). Visible absorbance -. 2. (or plate reader). No accessories are needed and SoftMax® Pro Software. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). measurements in compatible Molecular Devices® microplate readers. com. Time-resolved phosphorescence. The microplate was then placed in a SpectraMax. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. This can increase throughput and efficiency, thus saving time and space in the lab. The third tested COC-based plate, the UltraVision 384-well microplate, however, absorbed at 2 OD at 200 nm,. ©2013 Molecular Devices, LLC • 6/13 • Printed in USA • PN: 0120-1523. Automated live cell imaging – cell counting and confluence. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. Add the new plate. x. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Top. SpectraMax® M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. VMax . 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 030 fmol Fluorescence Polarization (FP). flat meniscus), but is approx 15 mL. If you have an xml plate object, then. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Figure 3. 19 H mm that meet microplate industry standards. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. Polypropylene deep well plates: Elevated well walls for easier sealing Round. A snapshot of the Settings screen is shown in Figure 2. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. For biochemical assays, the washer removes unbound material and unreacted reagents. 2. within each well. 48 W x 12. Turn the reader on and secure the USB cable. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. 1 fmol luciferase per well. Access this by going to. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . Microplate readers are commonly used in life science laboratories. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. 3860 N First Street. VMax . Outer wells aligned for proper viewing with FLIPR. Values are averages from 5 clean plates, each filled with 200 µL water/well. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. . 20 attomol ATP per well. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Fluid dispensing speed. For Raman excitation, a continuous-wave (CW) laser at 532 nm (Verdi V-18, Coherent) was used. The SpectraMax ABS Plus reader utilizes Molecular . x. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. For SpectraMax L or LMax II, LMax II 384 - no adapter used. 384 well polystyrene microplates have plate dimensions of 127. Assay setup for a 384-well low-volume plate. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Description: The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax ID5 Plate Reader (Molecular Devices). A rinse program is included for quick and easy daily. 313 reference gain check fail. Round well design with a solid black flat bottom2. 3 µl). The former have been the traditional domain of plate readers and great strides have been. Pick the best speed, sensitivity, and price for your needs. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. $542. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Shaking: Linear, orbital, and double orbital. 96-, 384-, and 1536-well microplates. A rinse program is included for quick and easy daily. Infinite possibilities. the iD5 reader’s filter system. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Summary of Contents for Molecular Devices SpectraMax M2. explore more. 58% for the detection module and 2. Dimensions 128 x 86 x 15 mm. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Three low volume 24-well bottom slides. A = Log 10 (I 0 /I). The calculated LOQ values were 0. #i3X) with: SoftMax Pro 7. 5–25 µl. Both readers were heated to 37ºC prior to setting up the assay. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wave-length range (spectral scan). Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. M5. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. VIS absorbance detection. FLIPR Calcium 4 Assay Kit. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 2. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. All stand-alone instrument functions can be controlled using the software. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. 96- and 384-well . The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 340 PC384. ability to use as little as 2˜µL samples with 24- or 64-well plates. A ten-fold serial dilution of purified luciferase in PBS with 0. 384-Well Fully Skirted Plates, Barcoded, including Armadillo 384-Well PCR plates. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. 315 can't find zero order. Molecular Devices, LLC. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Using Molecular Devices microplate readers and SoftMax. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 96-384. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. For more sample throughput, both readers can be easily. In addition, the iD5 reader measures TRF and FP and. x. Contact our support team with your questions regarding Molecular Devices products and applications. Artel MVS Bundle DM Std/800TSNB Reader Details. #3-000-510)Page 154: Ordering Parts And Accessories. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. or use the modified protocol below for the generation of NPCs (see Note 1). SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. U-bottom: 30-40 µl. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. 6-384 (96- and 384-well with pipetting) 96-well microplates. 190. Validated by Molecular Devices for the FLIPR reader. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. $913. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Temperature Control: Ambient +5C up to 42C. Read one sample or up to 384. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. USA. Application Area: Spectrophotometer for molecular biology. Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. Selects either 96-well or 384-well plate for a stand-alone read. Streamline GLP/GMP compliance. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 96-, 384-, and 1536. 7. After reading the ELISA plate on both readers, each standard curve was nearly identical. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. +1 800. RUO. 1 fmol luciferase per well. The plate reader optics are tunable for. Versa Max. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. 384- or 96-well read plate containing cells loaded. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. 616, 665 nm EM filters 0200-7011. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Molecular Devices: n/a:. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. 046 to 0. #ID5) HTRF Detection System (Molecular Devices cat. 96/384-well plate formats. ) 0. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. #3635) Transferpette micropipette (Drummond Scientific cat. minutes and begin reading microplates with seven mouse clicks. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. Selects either 96-well or 384-well plate for a stand-alone read. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Our microplate. EMax® Plus Microplate Reader, Molecular Devices Catalog Number: (10119-370) Supplier: Molecular Devices Description: Robust, high value microplate reader designed to. Time-resolved phosphorescence. 100 fmol 1536-well (8 µL) 0. No. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Service parts for these models are not being produced and are no longer in stock. Molecular Devices has provided scientists with tools to expand the boundaries of their research. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. B. That explains the lower values on the LmaxII384 plate reader. 96- and 384-well microplates. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. The same measurement settings can be used for subsequent plates as long as the volumes, tracer and concentrations remain the same. Two high-energy xenon flash lamps: Reading capabilities. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Reliable performance with a proven track record. UV absorbance detection. SpectraMax ID5 Plate Reader (Molecular Devices). Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells. 9. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. SpectraMax i3x. 1/17 1907B Printed in USA . Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. [Carbachol] (nM) RFU Max. Two different agonists, acetylcholine and carbachol, were tested. The plate reader consisted of a large-area. Dimensions: 18. Features. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. Published Dec 16, 2020 | Updated Dec 16, 2020. Fluorescence Microplate Reader Comparison Chart. Expand the Microplates tree. Molecular Devices has provided scientists with tools to expand the boundaries of their research. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. * Note: Programming the washer with a final aspirate height of 4. Loading Buffer was also added to a set of cell-free wells for background controls. #4TI-0655) Microplates. 2 with 1000 cells. Molecular Devices makes. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. These models can no longer be repaired. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. #W50-100). Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Products : Ionworks HT, Ionworks Quattro. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. 384-WELL SBS STERILE MICROPLATE. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Includes PC and software. 0 . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Description. flat-bottom: 50-60 µl. Read the microplate. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. EMax. EMax. 96-Deep Round or Square Well Plates. Alpha 384 HTS 96- and 384-well . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. . Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. ,LTD (CAT#: STEM-S. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. UV absorbance detection. Temperature control. 1 x plates. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. 003 fmol 0. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. the Molecular Devices SPECTRAmax® 190 and 250 plate. The FLIPR 384 system comes complete with 384. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. SpectraMax iD5. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 0 out of 5 $ 14,499. ". For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. x. 305 reference level saturation. 1. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Full-skirted standard or extra. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. For more sample throughput, the system canabsorbance plate readers. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. QuickSync. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). 384 well plate (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc. 5in (46. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Shaking: Linear and Orbital. Inquiry Basket. This kit is designed for customers who. Microplates for applications that require high sample recovery rates. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Instrument settings are listed in Table 1. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. highthroughput alphascreen, alphascreen on plate reader Created Date:SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Service Contracts, Preventative Maintenance and. Turn the. If an organoid line forms small organoids in the 24-well semi. plates < 100 amol (384-well) 1 0200-7018. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. 304 signal level saturation. External Dimensions without lid (mm). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. A. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 6-384. Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. FlexIII. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. x. 00. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. Alpha 1536 HTS 96, 384, and . 100 fmol 384-well (75 µL) 0.